Proposed experiments

• Determine region of interaction further :

• Agno: synthetic peptides
• PCNA : use the SHV43, QLGI125, VDK188 , point mutations (K63, K164, K127?)

• Replication assays :

• Using pol ± ¯¸ +/- Agno WT/mutants
• Proliferation assays ?

• DNA damage and repair

• Comet asssay, LM-PCR, gene/protein levels, cell viability assays