Host collaboration : part ii

• Poliovirus requires host proteins (in Xenopus) :

• • • Host cell poly(rC)-bi prot binds first 180 nt of +ssRNA of cloverleaf and facilitates bi of 3CD to opposite side of same cloverleaf
    • Poly(A)-bi prot interacts with poly(rC)bi prot 2 and 3CDpro and 3' poly-A tal of viral RNA (circular vRNA)