RNA synthesis

• • • 3CDpro :
    • • participates in formation of ribonucleoprot at end of 5' end of + strand
      • binds together with cellular protein “”poly(rC)-binding protein 2” to cloverleaf structure
      • RNA bi dom in 3C
    • Cis Acting Replication Elements (cre) e.g. polio and rhinovirus confer initiation specificity to RNA polymerase
    • VPg is primer for RNA synthesis => uridylylation in infected cells. Templates for uridylylation is an RNA hairpin, cisacting elet, i/t coding region