Mk5 - The Mission

• transgenic mice with MK5L337A

• stable cell lines containing the ca mutant

• promotor region is being cloned, sequenced: CRE element present

• PKA, PKC, PKD phosphorylate in vitro

  • residue numbers via Netphosp server (Denmark)
  • mutations of MK5 PKA sites to nonacceptor ala res
  • problems : GST steric hindrance ?