III. Genome-wide RNAi

• dsRNA libraries (Drosophila)

• • Flow :
  • • robotically aliquot dsRNA per well
    • robotically dispense cells
    • parallell analysis (after 3D)
    • • Transcriptional reporters (fluorescence), Ab based screens, FACS, Microscopy, Microarray
  • update libraries to address offtargets