Off-target effects

• • Concentration and kinetics analysis
  • • unable to titrate off-target gene regulation from silencing unintended targets (not due to high [])
  • Temporal gene expression patterns
  • • 4 groups :
    • • Group 1 : single transcript : p38
      • Group 2 : 9 transcripts ~ off-targets
      • Group 3 and 4 : genes altered after p38 (2nd effects)